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human liver tissue specimens  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology human liver tissue specimens
    Human Liver Tissue Specimens, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human liver tissue specimens/product/Santa Cruz Biotechnology
    Average 93 stars, based on 18 article reviews
    human liver tissue specimens - by Bioz Stars, 2026-05
    93/100 stars

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    Expression of CD24 in human HCC clinical specimens and cell lines. Notes: ( A ) Amino acid sequence alignment of CD24A with CD24B; ( B ) qPCR was carried out to evaluate the mRNA expression of CD24A in 90 pairs of HCC tissues and corresponding adjacent non-cancerous liver tissues; ( C ) Western blotting was performed to detect the expression of CD24 in 4 pairs of HCC tissues (K) and corresponding adjacent non-cancerous liver tissues (N); ( D ) mRNA expression levels of CD24A and CD24B in human HCC cell lines were detected by qPCR; ( E ) the protein expression level of CD24 in human HCC cell lines was determined by Western blotting; ( F ) the mRNA expression levels of CD24A and CD24B in SK-Hep1 and Li7 cells stably transfected with CD24A, CD24B or control (pWPXL) vectors; ( G ) Western blot analysis of CD24 protein in SK-Hep1 and Li7 cells stably transfected with CD24A, CD24B or control (pWPXL) vectors. ** P <0.01.

    Journal: OncoTargets and therapy

    Article Title: CD24 isoform a promotes cell proliferation, migration and invasion and is downregulated by EGR1 in hepatocellular carcinoma

    doi: 10.2147/OTT.S196506

    Figure Lengend Snippet: Expression of CD24 in human HCC clinical specimens and cell lines. Notes: ( A ) Amino acid sequence alignment of CD24A with CD24B; ( B ) qPCR was carried out to evaluate the mRNA expression of CD24A in 90 pairs of HCC tissues and corresponding adjacent non-cancerous liver tissues; ( C ) Western blotting was performed to detect the expression of CD24 in 4 pairs of HCC tissues (K) and corresponding adjacent non-cancerous liver tissues (N); ( D ) mRNA expression levels of CD24A and CD24B in human HCC cell lines were detected by qPCR; ( E ) the protein expression level of CD24 in human HCC cell lines was determined by Western blotting; ( F ) the mRNA expression levels of CD24A and CD24B in SK-Hep1 and Li7 cells stably transfected with CD24A, CD24B or control (pWPXL) vectors; ( G ) Western blot analysis of CD24 protein in SK-Hep1 and Li7 cells stably transfected with CD24A, CD24B or control (pWPXL) vectors. ** P <0.01.

    Article Snippet: Ninety paired human primary HCC and matched adjacent non-cancerous liver tissue specimens were obtained from Qidong Liver Cancer Institute (Qidong, China).

    Techniques: Expressing, Sequencing, Western Blot, Stable Transfection, Transfection, Control

    EGR1 is downregulated in HCC tissues and represses CD24A expression in human HCC cells. Notes: ( A ) mRNA expression of EGR1 in 90 pairs of HCC tissues and corresponding adjacent non-cancerous liver tissues was detected by qPCR; ( B ) mRNA expression of EGR1 in 50 pairs of HCC tissues and corresponding adjacent non-cancerous liver tissues in the TCGA database was analyzed; ( C ) Western blotting was performed to detect the expression of EGR1 in four pairs of HCC tissues (K) and corresponding adjacent non-cancerous liver tissues (N); ( D , E ) the expression of EGR1 in human HCC cell lines was assessed via qPCR and Western blotting; ( F ) qPCR (top panel) and Western blotting (bottom panel) analyses of EGR1 and CD24A in EGR1-overexpressing SMMC-7721 and Hep3B cells; ( G ) tumor xenografts were collected from BALB/c (nu/nu) mice inoculated with Li7 cell lines stably overexpressing EGR1 or control (pWPXL) vectors (left panel). Xenografts were weighed and analyzed (right panel); n=10; ( H ) the EGR1 protein level was detected by Western blotting in xenograft tissue samples. β-Actin was used as the loading control. * P <0.05; ** P <0.01.

    Journal: OncoTargets and therapy

    Article Title: CD24 isoform a promotes cell proliferation, migration and invasion and is downregulated by EGR1 in hepatocellular carcinoma

    doi: 10.2147/OTT.S196506

    Figure Lengend Snippet: EGR1 is downregulated in HCC tissues and represses CD24A expression in human HCC cells. Notes: ( A ) mRNA expression of EGR1 in 90 pairs of HCC tissues and corresponding adjacent non-cancerous liver tissues was detected by qPCR; ( B ) mRNA expression of EGR1 in 50 pairs of HCC tissues and corresponding adjacent non-cancerous liver tissues in the TCGA database was analyzed; ( C ) Western blotting was performed to detect the expression of EGR1 in four pairs of HCC tissues (K) and corresponding adjacent non-cancerous liver tissues (N); ( D , E ) the expression of EGR1 in human HCC cell lines was assessed via qPCR and Western blotting; ( F ) qPCR (top panel) and Western blotting (bottom panel) analyses of EGR1 and CD24A in EGR1-overexpressing SMMC-7721 and Hep3B cells; ( G ) tumor xenografts were collected from BALB/c (nu/nu) mice inoculated with Li7 cell lines stably overexpressing EGR1 or control (pWPXL) vectors (left panel). Xenografts were weighed and analyzed (right panel); n=10; ( H ) the EGR1 protein level was detected by Western blotting in xenograft tissue samples. β-Actin was used as the loading control. * P <0.05; ** P <0.01.

    Article Snippet: Ninety paired human primary HCC and matched adjacent non-cancerous liver tissue specimens were obtained from Qidong Liver Cancer Institute (Qidong, China).

    Techniques: Expressing, Western Blot, Stable Transfection, Control